Apparent steady-state kinetic constants of TmP2O wild-type and mutants with D-glucose or D-galactose as electron donor, and O2 (air) under saturation as electron acceptor.

aSince H167A was not designed to specifically improve galactose turnover, but to slow down flavin reduction during the reductive half-reaction, the kinetic constants for H167A were not determined. The mutant was included since it is a close structural mimic of the wild-type when sugar binding is concerned. Its purpose is therefore mainly for structural comparisons.鉴于H167A的设计初衷并非特异性提升半乳糖周转效率，而是旨在减缓还原半反应过程中的黄素还原速率，因此未对该突变体的动力学常数进行测定。该突变体之所以被纳入数据集，是因为就糖结合而言，其结构与野生型（wild-type）高度相似。因此，该突变体的主要用途为结构比对分析。