Self-grooming behavior of <em>Aedes aegypti</em> exposed to <em>Metarhizium humberi</em> formulation

Aedes aegypti L. (Diptera, Culicidae), the key vector of viral diseases to humans in the tropics, is susceptible to infection with entomopathogenic fungi. Self-grooming behavior, which has still been little studied in mosquitoes, may possibly interfere with fungal infection through the cuticle. In this study, self-grooming by A. aegypti adults exposed to Metarhizium humberi Luz, Rocha &amp; Delalibera 2019 (Hypocreales, Clavicipitaceae) conidia mixed or not with micronized carnauba wax (MCW) led to a decrease of non-germinated conidia and early germinating stages from their body surface up to 24 hours after exposure to a fungus-treated surface. Adults showed a low-level, spontaneous, and stimulus-independent self-grooming behavior that was distinctly increased after exposure to conidia mixed or not with MCW or solely to MCW. The first pair of legs scraped synchronously the proboscis, antennae, and wings; legs of the third pair scraped each other as well as synchronously the first and se..., , , # Self-grooming behavior of *Aedes aegypti* exposed to *Metarhizium humberi* formulation --- We have submitted 11 comma-separated value (CSV) data files documenting behavioral observations, fungal localization, and surface contamination related to self-grooming activity in *Aedes aegypti* (Diptera: Culicidae) adults exposed to *Metarhizium humberi* (Hypocreales: Clavicipitaceae) conidia, micronized carnauba wax (MCW), or their combination. The data capture the influence of fungal and wax treatments on grooming frequency, body-surface contamination, and conidial persistence over time. Each dataset corresponds to one experimental figure (Fig. 1–4) from the associated publication, subdivided by treatment type: *C* (conidia only), *C+MCW* (conidia + micronized carnauba wax), and *MCW* (micronized carnauba wax only). --- ## Descriptions ### **Fig_1_C.csv**, **Fig_1_C_MCW.csv**, **Fig_1_MCW.csv** These files contain behavioral observation data for *Aedes aegypti* adults following expos...,埃及伊蚊（*Aedes aegypti* L.，双翅目（Diptera）蚊科（Culicidae））是热带地区人类病毒性疾病的关键传播媒介，对昆虫病原真菌的侵染具有易感性。目前针对蚊子的自我梳理行为研究仍较为匮乏，该行为或可通过体壁干扰真菌侵染。本研究中，暴露于混有或未混有微化巴西棕榈蜡（micronized carnauba wax, MCW）的亨伯尔绿僵菌（*Metarhizium humberi* Luz, Rocha & Delalibera 2019，肉座菌目（Hypocreales）麦角菌科（Clavicipitaceae））分生孢子的埃及伊蚊成虫，其自我梳理行为可使体表未萌发的分生孢子及早期萌发阶段的孢子数量在暴露于真菌处理表面后的24小时内显著减少。埃及伊蚊成虫存在低水平、自发性且不依赖外界刺激的自我梳理行为，在暴露于混有或未混有MCW的分生孢子，或仅暴露于MCW后，该行为均显著增强。第一对足同步刮擦喙、触角及翅膀；第三对足则相互刮擦，同时同步刮擦第一对足及……，，，# *埃及伊蚊暴露于亨伯尔绿僵菌制剂的自我梳理行为* --- 我们共提交了11个逗号分隔值（comma-separated value, CSV）数据文件，记录了埃及伊蚊（双翅目：蚊科）成虫暴露于亨伯尔绿僵菌（*Metarhizium humberi*，肉座菌目：麦角菌科）分生孢子、微化巴西棕榈蜡（MCW）或二者混合制剂后的自我梳理行为、真菌定位及体表污染相关观测数据。 这些数据涵盖了真菌与蜡质处理对梳理频率、体表污染程度及分生孢子随时间存留情况的影响。每个数据集对应关联发表论文中的一幅实验图（图1至图4），并按处理类型细分：C（仅分生孢子）、C+MCW（分生孢子+微化巴西棕榈蜡）及MCW（仅微化巴西棕榈蜡）。 --- ## 数据文件描述 ### **Fig_1_C.csv**、**Fig_1_C_MCW.csv**、**Fig_1_MCW.csv** 这些文件包含埃及伊蚊成虫暴露后的行为观测数据……