Impact of Zika Virus Infection on Human Neural Stem Cell MicroRNA Signatures [array]

We used whole genome expression profiling to analyze the effects of Zika virus (ZIKV) strains Uganda and French Polynesia on the mRNA transcriptome of induced pluripotent stem cell-derived human neural stem cells (long-term self-renewing neuroepithelial-like stem (lt-NES®) cells) - particularly to identify differentially expressed genes which are associated with microcephaly phenotype. Gene expression profiles of Zika virus-infected lt-NES® cells were analyzed 24, 48 and 72 hours post infection. At the same timepoints gene expression profiles of uninfected lt-NES® cells were analyzed. 3 biological replicates were used.