A fungal endophyte alters poplar leaf chemistry, deters insect feeding, and shapes insect community assembly

The endophytic fungi of certain grasses and other herbaceous plants have long been known to provide plants with anti-herbivore defense compounds, but there is little information about whether the endophytes of trees also engage in such mutualisms. We investigated the influence of the endophytic fungus Cladosporium sp. on the chemical defenses of black poplar (Populus nigra) trees and the consequences for feeding preference and fitness of herbivorous insects and insect community assembly. Endophyte colonization increased both constitutive- and induced poplar defenses. Generalist Lymantria dispar larvae preferred and performed better on uninfected over endophyte-infected poplar leaves, most likely due to higher concentrations of salicinoids in endophytic leaves and the endophyte-produced alkaloid stachydrine. Under field conditions, the endophytic fungus also shapes insect community assembly in young black poplar trees. Our results show that endophytic fungi can play a major role both in ..., , , # Data from: A fungal endophyte alters poplar leaf chemistry, deters insect feeding, and shapes insect community assembly

Corresponding author

Sybille B. Unsicker [[e-mail]](mailto:[e-mail])

## General description

This README file describes the data package accompanying the above-mentioned publication.

Data used in the analysis are either

1. .xlsx and .csv files for R.scripts
2. R. scripts
3. Excel file with a summary of raw data and data used for statistics in spss
4. MSMS data as .mzML files

## Code/ software

1. .xlsx and .csv files for R.scripts R 4.3.2
2. R. scripts: Files can be opened using R 4.3.2
3. Excel file with a summary of raw data and data used for statistics in IBM SPSS statistics 25
4. MSMS data as .mzML files:Files can be opened using open software e.g., ProteoWizard or OpenMS.

## File list and description

.xlsx and .csv files for R.scripts

#### \"Walther et al. 2024\_volcano.xlsx\"

A file containing results of feature intens...

长期以来，学界已证实部分禾本科植物及其他草本植物的内生真菌（endophytic fungi）可为宿主合成抗草食动物防御化合物，但针对树木内生真菌是否也能建立此类共生关系的相关研究仍较为匮乏。本研究以欧洲黑杨（Populus nigra）为研究对象，探究了枝孢霉属（Cladosporium sp.）真菌对其化学防御的调控作用，以及该调控对草食昆虫取食偏好、适合度与昆虫群落构建的影响。

内生真菌定殖可同时提升欧洲黑杨的组成型防御与诱导型防御能力。广食性舞毒蛾（Lymantria dispar）幼虫更偏好取食未被内生真菌定殖的黑杨叶片，且在该类叶片上的生长表现更佳，这一现象大概率源于被内生真菌定殖的叶片中水杨苷类化合物浓度更高，同时存在内生真菌合成的生物碱水苏碱。在野外环境中，该内生真菌同样会对幼年欧洲黑杨的昆虫群落构建过程产生调控作用。本研究结果表明，内生真菌在……中均可发挥关键作用。
# 数据来源：《真菌内生真菌改变杨树叶片化学组成、抑制昆虫取食并调控昆虫群落构建》

## 通讯作者
西比尔·B·翁西克（Sybille B. Unsicker） <[e-mail]>

## 总体说明
本自述文件用于说明上述配套发表论文的数据包。

本研究分析所用数据包括以下四类：
1. 用于R脚本（R scripts）的.xlsx与.csv格式文件
2. R脚本文件
3. 包含原始数据汇总及用于IBM SPSS Statistics 25统计分析数据的Excel文件
4. 格式为.mzML的串联质谱（MSMS）数据

## 代码与软件
1. 用于R脚本的.xlsx和.csv文件，适配R 4.3.2版本
2. R脚本文件：可使用R 4.3.2版本打开
3. 包含原始数据汇总及用于IBM SPSS Statistics 25统计分析数据的Excel文件
4. 格式为.mzML的串联质谱（MSMS）数据：可使用ProteoWizard、OpenMS等开源软件打开

## 文件列表与说明
### 用于R脚本的.xlsx与.csv格式文件
#### "Walther et al. 2024_volcano.xlsx"
该文件包含特征强度分析结果……