Figure S1 - Combination Effect of Epigenetic Regulation and Ionizing Radiation in Colorectal Cancer Cells

Experimental procedures for this study. Human colorectal carcinoma cell lines (HCT116, SW480 and DKO) were seeded and treated with 5-aza-dC (0.5 or 1 µM) for 72 h prior to irradiation with gamma rays (2, 5, or 10 Gy). Twenty-four hours after irradiation, the cells were assayed for colony formation, cell proliferation, cell viability, tumor growth, cell cycle, annexin V, and the activity levels of caspases 3 and 7. In addition, comet assays and western blots were also performed. (DOCX)本研究的实验流程如下：将人类结直肠癌细胞系（HCT116、SW480及DKO）接种培养后，采用5-氮杂-2'-脱氧胞苷（5-aza-dC，浓度设置为0.5 μM或1 μM）处理72小时，随后以γ射线（辐射剂量分别为2 Gy、5 Gy、10 Gy）进行辐照。辐照后24小时，对细胞进行集落形成能力、细胞增殖活性、细胞活力、肿瘤生长特性、细胞周期、膜联蛋白V（annexin V）以及半胱天冬氨酸蛋白酶3和7的活性水平检测。此外，本研究还开展了彗星实验（comet assay）与蛋白质印迹（western blot）实验。（DOCX）