Bacterial mock community analysis using Nanopore long-read metabarcoding

A mock community made of 17 bacterial species was assembled so that each species is represented by the same number of ribosomal operon sequences in the mix. This mock community was then used in a metabarcoding analysis using Nanopore long-read sequencing. Four primer sets targeting the ribosomal operon region were tested, and two different sequencing chemistries were used. The generated sequencing datasets were processed with the PRONAME pipeline, which allowed demonstrating how this pipeline can correct sequencing errors and significantly increase sequence accuracy.