segmentations of mouse trabeculae

Grey scale image stacks (TIFF) of segmented trabecular bone from below the proximal growth plate of both tibiae obtained from 12 female C57BL/J6 mice.All mice underwent sciatic neurectomy to their right hindlimb at 12 weeks of age, to model osteoporosis.Four mice were euthanised and their bones dissected after each of 3 time points: 5,35, and 65 days after the surgery. All tibiae were imaged in X-ray microcomputed tomograph (Skyscan 1172, Bruker microCT, Kontich, Belgium) at 5 µm voxel size and grey scale images were reconstructed using NRecon 1.7.1.0.File names reflect the mouse ID, timepoint of euthanasia and whether the sample is from the left or right hindlimb, so e.g. the file 'mouse-35.3R-segmented-grey-scale' is from the right hindlimb of mouse number 3 of the ones that were taken 35 days post-surgery (so at 12 weeks + 35 days of age).Segmentation was done manually using CTan.Ethical approval for the animal procedures was carried out in accordance with the Animals (Scientific Procedures) Act 1986, an Act of Parliament of the United Kingdom, approved by the Royal Veterinary College Ethical Review Committee and the United Kingdom Government Home Office under specific project license, and followed ARRIVE (Animal Research: Reporting of In Vivo Experiments) guidelines.本数据集包含12只雌性C57BL/J6小鼠双侧胫骨近端生长板下方的分割小梁骨灰度图像栈（TIFF格式）。所有小鼠于12周龄时对右侧后肢实施坐骨神经切断术以构建骨质疏松症模型。在术后5天、35天和65天三个时间点，分别有4只小鼠被安乐死并取出骨骼。所有胫骨均通过X射线显微计算机断层扫描仪（Skyscan 1172，布鲁克显微CT（Bruker microCT），比利时孔蒂希）进行成像，体素尺寸为5 µm，使用NRecon 1.7.1.0软件重建得到灰度图像。文件名包含小鼠编号、安乐死时间点以及样本取自左侧还是右侧后肢的信息，例如文件"mouse-35.3R-segmented-grey-scale"对应的样本为术后35天（即小鼠年龄为12周龄加35天）的第3只小鼠的右侧后肢骨骼。骨骼分割采用CTan软件手动完成。本动物实验操作已按照英国1986年《动物（科学程序）法案》（英国议会法案）开展伦理审批，获得英国皇家兽医学院伦理审查委员会以及英国政府内政部的特定项目许可，并遵循ARRIVE (Animal Research: Reporting of In Vivo Experiments)指南。