RNA-sequencing of inguinal white adipose tissue (iWAT) from kynurenine 3-monooxygenase (Kmo) knockout mice and wild-type littermates on chow and high-fat diets.

To investigate if chronically elevated circulating kynurenic acid (KYNA) and reduced quinolinic acid (QUIN) levels affect whole-body energy metabolism, we used a knockout-first gene targeting strategy to eliminate Kmo gene expression in mice. We conducted a series of metabolic assessments on male and female mice fed either a chow or high-fat diet (HFD) and performed RNA-sequencing on the inguinal white adipose tissue (iWAT) from male Kmo-defficient mice and their wild-type littermate controls on chow and high-fat diets. Overall design: RNA sequencing analysis of inguinal white adipose tissue from whole-body male Kmo knockout mice and wild-type littermate controls on chow and high-fat diets (n = 4, age = 6-7 months). Total RNA was extracted using TRI reagent (Sigma-Aldrich, T9424) according to the manufacturer's instructions. RNA was further purified, and DNase treated (Zymo, R1013), and integrity confirmed prior to sequencing (Agilent Technologies, 2100 Bioanalyzer), with an RNA integrity number (RIN) greater than 7 as a threshold. Samples were purified by poly-A enrichment before library preparation using the Illumina NovaSeq X Plus Series platform with a paired-end 150 base pair strategy.