TSFv2,0

Database to be used with Rpsblastp+ to classify secreted tick salivary proteins.TickSialoFam (TSFam): A Database That Helps to Classify Tick Salivary Proteins, a Review on Tick Salivary Protein Function and Evolution, With Considerations on the Tick Sialome Switching Phenomenon. (2020) Ribeiro JM, Mans BJ. Frontiers in cellular and infection microbiology. 2020 July; doi: 10.3389.TickSialoFam 2.0 : A Deeper Insight into the Tick Salivary Protein Families under the Light of Alphafold2 and Dali: Introducing the TickSialoFam 2.0 Database. (2022) Mans BJ, Andersen JF, Ribeiro JMC. Int J Mol Sci. 2022 Dec 9;23(24). pii: 15613. doi: 10.3390/ijms232415613.Hard ticks feed for several days or weeks on their hosts and their saliva contains thousands of polypeptides belonging to dozens of families, as identified by salivary transcriptomes. Comparison of the coding sequences to protein databases helps to identify putative secreted proteins and their potential functions, directing and focusing future studies, usually done with recombinant proteins that are tested in different bioassays. However, many families of putative secreted peptides have a unique character, not providing significant matches to known sequences. The availability of the Alphafold2 program, which provides in silico predictions of the 3D polypeptide structure, coupled with the Dali program which uses the atomic coordinates of a structural model to search the Protein Data Bank (PDB) allows another layer of investigation to annotate and ascribe a functional role to proteins having so far being characterized as “unique”. In this study, we analyzed the classification of tick salivary proteins under the light of the Alphafold2/Dali programs, detecting novel protein families and gaining new insights relating the structure and function of tick salivary proteins.适用于Rpsblastp+以分类蜱分泌唾液蛋白的数据库。 TickSialoFam（TSFam）：蜱唾液蛋白分类数据库——一篇关于蜱唾液蛋白功能与演化的综述，并探讨蜱唾液组转换现象。(2020) Ribeiro JM、Mans BJ，发表于《细胞与感染微生物学前沿》（Frontiers in cellular and infection microbiology），2020年7月；DOI: 10.3389。 TickSialoFam 2.0：基于AlphaFold2与Dali的蜱唾液蛋白家族深度解析——TickSialoFam 2.0数据库介绍。(2022) Mans BJ、Andersen JF、Ribeiro JMC，发表于《国际分子科学杂志》（Int J Mol Sci），2022年12月9日；第23卷第24期，文章编号pii: 15613，DOI: 10.3390/ijms232415613。 硬蜱会在宿主体表取食数日至数周，其唾液中包含经唾液转录组学鉴定得到的、隶属于数十个家族的数千条多肽链。将编码序列与蛋白质数据库比对，可鉴定推定分泌蛋白并预测其潜在功能，为后续研究（通常通过重组蛋白开展不同生物测定实验）指明方向并聚焦研究重点。然而，诸多推定分泌肽家族具有独特性，无法与已知序列获得显著匹配。AlphaFold2程序可实现多肽三维结构的计算机模拟预测，结合使用结构模型原子坐标检索蛋白质数据库（Protein Data Bank, PDB）的Dali程序，可为此前被归类为“独特”的蛋白提供新的注释维度，并赋予其功能推测依据。本研究基于AlphaFold2与Dali程序分析了蜱唾液蛋白的分类，发现了新的蛋白家族，并对蜱唾液蛋白的结构与功能关联获得了全新认知。