Dissolved oxygen sensing using an optical fibre long period grating coated with hemoglobin

A method for the preparation of a sensor consisting of an optical fibre long period grating coated with human hemoglobin is described. The utility of this sensor in detecting dissolved oxygen in phosphate buffered saline solution, by the conversion of the coated hemoglobin from deoxyhemoglobin to oxyhemoglobin, is described. The sensor shows good repeatability with a %CV of less than 1% for oxygenated and deoxygenated states and no drift or hysteresis with repeated cycling.本文报道了一种传感器的制备方法：该传感器由涂覆人血红蛋白（human hemoglobin）的光纤长周期光栅（optical fibre long period grating）构成。本文同时阐述了该传感器在磷酸盐缓冲盐水（phosphate buffered saline）中检测溶解氧的应用原理：通过使涂覆的血红蛋白从脱氧血红蛋白（deoxyhemoglobin）转化为氧合血红蛋白（oxyhemoglobin），实现对溶解氧的检测。该传感器具备优异的重复性，在氧合与脱氧状态下的变异系数百分比（%CV）均低于1%，且经多次循环测试后无信号漂移或滞后现象。