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Osteoblasts remotely supply lung tumors with cancer-promoting SiglecFhigh neutrophils

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NIAID Data Ecosystem2026-03-12 收录
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RNAseq (3'DGE) profiles of osteoblasts from four lung cancer-bearing mice and three tumor-free mice. Osteoblasts were FACS-sorted using the following markers: CD45-CD31-Terr119-GFP+ from lineage depleted bone and bone marrow tissue of lung tumor-bearing or tumor-free age-, sex- and litter-matched KrasLSL-G12D/WT;p53Flox/Flox (KP)-Ocn GFP mice. Total RNA was prepared using the Trizol method followed cDNA preparation, amplification, Illumina adapter ligation and 3'end sequencing by Illumina HiSeq 2500

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2021-07-25
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