Figure 3 Source data

Figure 3. Oil Red Accumulation by 3T3-L1 Adipocytes is Unchanged by MCH. 3T3-L1 preadipocytes in 24-well plates were differentiated in the presence or absence of up to 100 nM MCH. On the 10th day of the differentiation protocol, cells were fixed and differentiated cells stained with Oil Red, which was then eluted with 200 ml isopropanol and A570 measured.图3 黑素浓缩激素（MCH）对3T3-L1脂肪细胞的油红染色蓄积量无影响。将接种于24孔板中的3T3-L1前体脂肪细胞，在添加或不添加最高浓度为100 nM的MCH的条件下完成分化诱导。于分化方案的第10天固定细胞，对已分化成熟的脂肪细胞进行油红染色，随后使用200 mL异丙醇洗脱结合的染色剂，并测定570 nm波长处的吸光度（A570）。