Comparing methods of human precision cut tissue slice culture - tissue slice viability and functional markers data

This study examines the viability and functionality of hPCTS using different tissue culture formats. hPCTS were cultured for 15 days using an organotypic polytetrafluoroethylene Millicell insert, a CELLBLOKS® hydrophilic polyethylene terephthalate flow system plate or without an insert. Viability was assessed through MTS assays, while functionality was determined by measuring urea and albumin secretion across the 15 days in culture.1. File Name: Viability Data NC3Rs paper.xlsx - Visibility: Anyone - Content type: Data - Description: MTS viability data for human precision cut tissue slices (hPCTS) cultured over 15 days using three different culture methods (no insert, Millicell insert, CELLBLOCKS®). - Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet - File size: ~51 kB - License: Creative Commons Attribution-Noncommercial-Share Alike 4.0 2. File Name: Urea and Albumin Data NC3Rs paper.xlsx - Visibility: Anyone - Content type: Data - Description: Percentage change in urea and albumin secretion into media from day 3 in hPCTS cultured over 15 days. - Mime-Type: application/vnd.openxmlformats-officedocument.spreadsheetml.sheet - File size: ~19 kB - License: Creative Commons Attribution-Noncommercial-Share Alike 4.0本研究探究了人类精准切片组织（human precision cut tissue slices, hPCTS）在不同组织培养体系下的存活活性与功能表现。研究采用器官型聚四氟乙烯Millicell插入式培养载体、CELLBLOKS®亲水性聚对苯二甲酸乙二醇酯流动系统培养板，以及无插入式培养三种方式，对hPCTS开展为期15天的体外培养。通过MTS检测法评估其细胞存活活性，并通过定量检测培养周期内（15天）尿素与白蛋白的分泌水平，衡量其组织功能状态。 1. 文件名称：Viability Data NC3Rs paper.xlsx - 可见性：公开可访问 - 文件类型：数据文件 - 文件描述：针对采用三种不同培养方式（无插入式培养、Millicell插入式培养、CELLBLOCKS®培养）体外培养15天的人类精准切片组织（hPCTS）的MTS存活活性检测数据 - MIME类型：application/vnd.openxmlformats-officedocument.spreadsheetml.sheet - 文件大小：约51 kB - 授权协议：知识共享署名-非商业性使用-相同方式共享 4.0 （Creative Commons Attribution-Noncommercial-Share Alike 4.0） 2. 文件名称：Urea and Albumin Data NC3Rs paper.xlsx - 可见性：公开可访问 - 文件类型：数据文件 - 文件描述：针对体外培养15天的人类精准切片组织（hPCTS），自培养第3天起，其培养基中尿素与白蛋白分泌量的百分比变化数据 - MIME类型：application/vnd.openxmlformats-officedocument.spreadsheetml.sheet - 文件大小：约19 kB - 授权协议：知识共享署名-非商业性使用-相同方式共享 4.0 （Creative Commons Attribution-Noncommercial-Share Alike 4.0）