Main components of ethyl acetate extract of Chimonanthus salicifolius and its effects on intestinal mucositis in mice induced by 5-fluorouracil

Abstract This study aimed to analyze the main components of ethyl acetate extract of Chimonanthus salicifolius (EAECS) and investigate its effect on intestinal mucositis in mice induced by 5-fluorouracil. EAECS was prepared, and its main components were analyzed. Fifty mice were randomly divided into control, model and 3, 6 and 9 g/kg EAECS groups. The latter three groups were intragastrically administrated with 3, 6 and 9 g/kg EAECS for 7 days, respectively. The intestinal mucositis model was established in latter four groups from the 1st to 7th day of administration. Results showed that, the main components in EAECS were rutin, scopolamine, isofraxidin, scoparone, quercetin and kaempferol. After administration, compared with model group, in 9 g/kg EAECS group the body weight was increased; the serum tumor necrosis factor α (TNF-α) and interleukin 6 levels were decreased, and the interleukin 10 level was increased; the intestinal tissue superoxide dismutase level was increased, and the malondialdehyde and myeloperoxidase levels wer decreased; the liver tissue TNF-α level was significantly decreased (all P摘要 本研究旨在分析柳叶腊梅（Chimonanthus salicifolius）乙酸乙酯提取物（ethyl acetate extract of Chimonanthus salicifolius, EAECS）的主要成分，并探讨其对5-氟尿嘧啶（5-fluorouracil）诱导的小鼠肠黏膜炎的干预作用。本研究制备了EAECS并对其主要成分进行分析。将50只小鼠随机分为对照组、模型组以及3、6、9 g/kg EAECS给药组。后三个给药组分别以对应剂量灌胃给予EAECS，连续给药7天。于给药第1至7天，为后四组（模型组及三个给药组）构建5-氟尿嘧啶诱导的小鼠肠黏膜炎模型。结果显示，EAECS中的主要成分为芦丁（rutin）、东莨菪碱（scopolamine）、异秦皮啶（isofraxidin）、茵陈素（scoparone）、槲皮素（quercetin）与山奈酚（kaempferol）。给药后，与模型组相比，9 g/kg EAECS组小鼠体重显著升高；血清肿瘤坏死因子α（tumor necrosis factor α, TNF-α）与白细胞介素6（interleukin 6, IL-6）水平显著降低，白细胞介素10（interleukin 10, IL-10）水平显著升高；肠组织中超氧化物歧化酶（superoxide dismutase, SOD）水平升高，丙二醛（malondialdehyde, MDA）与髓过氧化物酶（myeloperoxidase, MPO）水平显著降低；肝脏组织TNF-α水平显著降低（所有P