Table S1 - Thermostable DNA Polymerase from a Viral Metagenome Is a Potent RT-PCR Enzyme
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Exonuclease Assay. The indicated number of units of enzyme were incubated with [33P]-labeled PCR product for 10 minutes at 70°C as described in methods. Shown are the percent counts released with background (water-only control) subtracted. Not detected is indicated when counts are not significantly above background counts, i.e. (DOC)
外切核酸酶实验(Exonuclease Assay):按照实验方法所述,将指定活性单位的酶与[33P]标记的聚合酶链式反应(Polymerase Chain Reaction, PCR)产物在70℃条件下孵育10分钟。所得结果为扣除背景(仅水对照)后的释放计数百分比。当计数结果未显著高于背景计数时,标注为「未检出」,即(DOC)。
创建时间:
2015-12-02



