Supporting Information S1 - Early Response of Protein Quality Control in Gills Is Associated with Survival of Hypertonic Shock in Mozambique tilapia

Figure S1, Dot-blot analysis of the levels of ubiquitin-conjugated proteins in gills of tilapia directly transferred from fresh water (FW) to 20‰ seawater (SW). (A) Ubiquitin-conjugated protein levels were shown as relative values based on dot intensities. (B) Ponceau S total protein stain of blots was used as loading control. (C) The levels of ubiquitin-conjugated proteins increased significantly at 24 h post-transfer from FW to 20‰ SW. The asterisk indicated a significant difference (PFigure S2, Dot-blot analysis of the levels of ubiquitin-conjugated protein in gills of tilapia directly transferred from fresh water (FW) to 30‰ seawater (SW). (A) Ubiquitin-conjugated protein levels were shown as relative values based on dot intensities. (B) Ponceau S total protein stain of blots was used as loading control. (C) No significant difference was found in the levels of ubiquitin-conjugated proteins when tilapia were transferred from FW to 30‰ SW. Values are mean ± S.E.M (n = 5). (DOC)