Supporting Information S1 - Early Response of Protein Quality Control in Gills Is Associated with Survival of Hypertonic Shock in Mozambique tilapia

Figure S1, Dot-blot analysis of the levels of ubiquitin-conjugated proteins in gills of tilapia directly transferred from fresh water (FW) to 20‰ seawater (SW). (A) Ubiquitin-conjugated protein levels were shown as relative values based on dot intensities. (B) Ponceau S total protein stain of blots was used as loading control. (C) The levels of ubiquitin-conjugated proteins increased significantly at 24 h post-transfer from FW to 20‰ SW. The asterisk indicated a significant difference (PFigure S2, Dot-blot analysis of the levels of ubiquitin-conjugated protein in gills of tilapia directly transferred from fresh water (FW) to 30‰ seawater (SW). (A) Ubiquitin-conjugated protein levels were shown as relative values based on dot intensities. (B) Ponceau S total protein stain of blots was used as loading control. (C) No significant difference was found in the levels of ubiquitin-conjugated proteins when tilapia were transferred from FW to 30‰ SW. Values are mean ± S.E.M (n = 5). (DOC)补充图S1：罗非鱼从淡水（FW）直接转移至20‰海水（SW）后，其鳃组织中泛素结合蛋白（ubiquitin-conjugated proteins）水平的斑点印迹（Dot-blot）分析。(A) 泛素结合蛋白水平以斑点灰度强度为基准，以相对值呈现。(B) 采用丽春红S（Ponceau S）对印迹膜进行总蛋白染色，作为上样内参。(C) 罗非鱼从淡水转移至20‰海水后24小时，其鳃组织中泛素结合蛋白水平显著升高，星号标注表示差异具有统计学意义（P）。补充图S2：罗非鱼从淡水直接转移至30‰海水（SW）后，其鳃组织中泛素结合蛋白水平的斑点印迹分析。(A) 泛素结合蛋白水平以斑点灰度强度为基准，以相对值呈现。(B) 采用丽春红S对印迹膜进行总蛋白染色，作为上样内参。(C) 罗非鱼从淡水转移至30‰海水后，其鳃组织中泛素结合蛋白水平未出现显著差异。数据以平均值±标准误（mean ± S.E.M）表示，样本量n=5。（DOC文件）