The levels of human PDAC microRNAs’ expression in 10 patients with longest survival and 10 with shortest survival were selected

MicroRNAs can be used in the prognosis of malignancies; however, their regulatory mechanisms are unknown, especially in pancreatic ductal adenocarcinoma (PDAC).From the 120 PDAC patients who underwent surgical resection, 10 patients with longest survival and 10 with shortest survival were selected. We found that high miR-29b-2-5p expression was associated with good prognosis (p=0.02). The validation cohort confirmed miR-29b-2-5p as an independent prognostic factor in PDAC (n=100, 95%CI=0.305-0.756, p=0.002). Among the 120 cases, 20 cases with similar clinicopathologic features and treatment but considerable different outcomes, composed the initial screening cohort for the miRNA microarray. What’s more, half of the patients had anextremely poor prognosis dying within the first year of diagnosis and were classified as “poor prognosis”, and the other 10 cases survived more than 21 months, which were classified as “good prognosis”.