Relative receptor affinities, metabolic and mitogenic potencies, and IR off-rates [% of human insulin].

All assays were performed in at least three independent experiments. Data are means ± SD and presented relative to human insulin. For human insulin, IR assay IC50 values were in the picomolar affinity range, IGF-IR assay IC50 values were in the nanomolar affinity range, rFFC assay EC50 values were in the picomolar range, and mitogenic assay EC50 values were in the nanomolar range (HMECs) and low nanomolar range (L6-hIR). The dissociation rate constant for human insulin was (3.7±0.3×10−2 min−1).所有检测实验均至少独立重复三次。实验数据以平均值±标准差（SD）形式呈现，并以人胰岛素为参照进行归一化处理。针对人胰岛素而言，胰岛素受体（IR）检测的半最大抑制浓度(IC50)处于皮摩尔级亲和力区间，胰岛素样生长因子1受体(IGF-IR)检测的半最大抑制浓度(IC50)处于纳摩尔级亲和力区间；重组FFC(rFFC)检测的半最大有效浓度(EC50)处于皮摩尔级区间，增殖检测的半最大有效浓度(EC50)在人乳腺上皮细胞(HMECs)中为纳摩尔级范围，在转染人胰岛素受体的L6细胞(L6-hIR)中为低纳摩尔级范围。人胰岛素的解离速率常数为(3.7±0.3)×10^-2 min^-1。