Time series data of microbial communities

Time series abundances of bacterial prey species and their morphotypes appearing in time with and without a protist predator species. All microcosms were started with equal abundance for all prey species either with or without the predator (See "Notes" section in the data file for the descriptions of species). Static microcosms consisted of 20 mL Brunner-CR2 medium in 50-mL cell culture flasks, incubated at 25°C. Prey and predator numbers were adjusted in the experimental medium to 1.8 x 107 cells mL-1 and 4.2 x 104 cells mL-1, respectively. During the experiment, we performed daily 10-fold dilutions by transferring 10% of the community into fresh medium. All microcosms were sampled 32 times (from 3 to 34 days ). All bacterial species were quantified by plating on Brunner CR-2 medium. All visible, differently coloured colonies were recorded after 2 days of incubation at 25°C using different dilutions from 103 to 108. Cell numbers of protist predator were estimated by counting cells fixed with 0.2% Lugol’s iodine solution under an inverted microscope with a counting chamber.本数据集包含原生生物捕食者（protist predator）存在与不存在条件下，细菌猎物物种及其形态型的时间序列丰度数据。所有微生物微宇宙培养体系（microcosm）均以各猎物物种初始丰度一致的条件启动，无论是否添加捕食者（详见数据文件"注释"章节的物种描述信息）。静态微生物微宇宙培养体系采用50mL细胞培养瓶盛装20mL Brunner-CR2培养基，于25℃条件下静置孵育。实验初始时，将猎物细菌与原生生物捕食者的浓度分别调整至1.8×10^7 细胞·mL⁻¹与4.2×10^4 细胞·mL⁻¹。实验期间，每日取10%的群落培养液转接至新鲜培养基中，完成10倍梯度稀释操作。所有培养体系均进行了32次采样，采样时间跨度为第3天至第34天。所有细菌物种均通过涂布法定量：将样品按10³至10⁸的稀释梯度涂布于Brunner CR-2培养基平板，于25℃孵育2天后，记录所有可见的异色菌落。原生生物捕食者的细胞数量通过以下方式估算：使用0.2%卢戈氏碘液（Lugol’s iodine solution）固定细胞后，在配备计数池的倒置显微镜下进行细胞计数。