Supplementary Data: How to eliminate scabies parasites from fomites – a high throughput ex vivo experimental study.

Research Hypothesis: To date, no field studies have been performed to validate decontamination measures for scabies infested textiles. As there is no in vitro culture, previous experimental research was limited in parasite numbers and did not consider the egg stage. Accordingly, the existing decontamination protocols are based on poor statistical power and vary significantly, resulting in confusion of patients and medical workers. We aimed to generate statistically valid experimental data as a basis to develop standardized guidelines with clear and simple directions for scabies outbreaks.
Over 5,500 adult mites and 2,300 eggs of S. scabiei were obtained from a porcine model, exposed to 89 different conditions, and individually monitored in custom designed wire mesh pouches (video) in experimental groups of 20-25. Initial in vitro mite survival and egg hatching assays (Supplemental Table 1) informed subsequent real life ex vivo experiments (Supplemental Table 2), resulting in a simple strategic flowchart for decontamination.

研究假设：截至目前，尚无实地研究可用于验证疥疮污染纺织品的净化措施。由于无法开展体外培养，既往实验研究的寄生虫样本数量受限，且未考虑虫卵阶段。因此，现有净化方案基于统计效力不足的研究，且各方案间差异显著，给患者与医务工作者造成了困惑。本研究旨在生成具备统计有效性的实验数据，以此为基础制定针对疥疮暴发事件的清晰、简易的标准化净化指南。
本研究从猪模型中获取了超过5500只成年疥螨（S. scabiei）与2300枚虫卵，将其暴露于89种不同处理条件下，并在定制设计的网袋（附带视频）中以20~25个样本为一组进行单独监测。初始的体外螨存活与虫卵孵化实验（补充表1）为后续的贴近真实场景的离体实验（补充表2）提供了依据，最终形成了一套简易的净化策略流程图。