Proteins that co-immunoprecipitate with CrCDKL5.

All Proteins: a list of all proteins identified in three technical repeats of each of two immunoprecipitation experiments (MS Experiment 10 and MS Experiment 11) with each protein’s total precursor intensity reported for each repeat. “#N/A” means that the protein was not identified in that experiment. Calculation of ratio and P value is described in the Materials and methods section. “Yes” in “Positive” columns means that the protein met the following criteria: the ratio of total precursor intensity in the experimental sample (lf5 CDKL5-GFP-TG) to that in the control sample (lf5) was larger than 10, and the P value was smaller than 0.01. CrCDKL5-GFP was the bait protein. Human keratin proteins and catalase were included in the sequence database to monitor contamination and to improve the accuracy and reliability of the MS protein identifications. Positive Proteins: a list of proteins that were positive in both immunoprecipitation experiments with their total precursor intensities reported for each technical repeat. In addition to the bait proteins (CrCDKL5-GFP and GFP), 6 proteins were identified. Interestingly, four of them as well as CrCDKL5 are among the 155 ciliary proteins reported to be methylated by King and colleagues [47]. PMID: 38696262. The proteins are sorted according to their lf5 CDKL5-GFP-TG to lf5 ratios in Experiment 10. (XLSX)