Proteins that co-immunoprecipitate with CrCDKL5.

All Proteins: a list of all proteins identified in three technical repeats of each of two immunoprecipitation experiments (MS Experiment 10 and MS Experiment 11) with each protein’s total precursor intensity reported for each repeat. “#N/A” means that the protein was not identified in that experiment. Calculation of ratio and P value is described in the Materials and methods section. “Yes” in “Positive” columns means that the protein met the following criteria: the ratio of total precursor intensity in the experimental sample (lf5 CDKL5-GFP-TG) to that in the control sample (lf5) was larger than 10, and the P value was smaller than 0.01. CrCDKL5-GFP was the bait protein. Human keratin proteins and catalase were included in the sequence database to monitor contamination and to improve the accuracy and reliability of the MS protein identifications. Positive Proteins: a list of proteins that were positive in both immunoprecipitation experiments with their total precursor intensities reported for each technical repeat. In addition to the bait proteins (CrCDKL5-GFP and GFP), 6 proteins were identified. Interestingly, four of them as well as CrCDKL5 are among the 155 ciliary proteins reported to be methylated by King and colleagues [47]. PMID: 38696262. The proteins are sorted according to their lf5 CDKL5-GFP-TG to lf5 ratios in Experiment 10. (XLSX)全部蛋白质：涵盖两项免疫沉淀（immunoprecipitation）实验（质谱实验10与质谱实验11）各三次技术重复中鉴定得到的所有蛋白质，报告了每一次技术重复下各蛋白质的总前体离子强度。"#N/A"表示该蛋白质在对应实验中未被鉴定到。比值与P值的计算方法详见材料与方法章节。"阳性"列中的"Yes"代表该蛋白质满足以下条件：实验样本（lf5 CDKL5-GFP-TG）与对照样本（lf5）的总前体离子强度比值大于10，且P值小于0.01。CrCDKL5-GFP为诱饵蛋白（bait protein）。序列数据库中纳入人类角蛋白与过氧化氢酶，用于监测实验污染并提升质谱（Mass Spectrometry，MS）蛋白质鉴定的准确性与可靠性。 阳性蛋白质：涵盖在两项免疫沉淀实验中均呈阳性的蛋白质，报告了各技术重复中的总前体离子强度。除诱饵蛋白（CrCDKL5-GFP与GFP）外，共鉴定到6种蛋白质。值得注意的是，其中4种蛋白质与CrCDKL5一同被归入King及其同事[47]报道的155种甲基化纤毛蛋白质之列。PMID: 38696262。本数据集的蛋白质按照实验10中lf5 CDKL5-GFP-TG与lf5的比值进行排序。(XLSX)