PRRX1 overexpression in MDA-MB-231 breast cancer cells

To investigate downstream targets of PRRX1, we used MDA-MB-231 (MDA231) breast cancer cells which express low level of PRRX1 to generate a stable cell line where human PRRX1 was ectopically overexpressed (MDA231-PRRX1), and performed comparative microarray analyses. Interestingly, we found many miRNAs that were upregulated in MDA231-PRRX1 cells. MDA231 cells were transduced with lentiviral vectors containing PRRX1, GFP and puromycine resistance cassette, and were selected with antibiotics. Pool of stably transduced cells were used for further analyses and microarray.