File S1 - Effects of Candesartan on Electrical Remodeling in the Hearts of Inherited Dilated Cardiomyopathy Model Mice

Figure S1. Effects of hydralazine on ECG data. A. Typical traces of ECG from WT and Hydralazine treated DCM mice. B. Comparison of QRS (upper panel) and QTc (lower panel) intervals. Data with WT, untreated control and candesartan-treated DCM mice are same as those in Fig. 2B. n = 7 for hydralazine. *P†PFigure S2. Effects of candesartan treatment on expression levels of mRNA encoding K+ channels and accessory subunit in WT left ventricle. Quantitative real-time PCR analysis was carried out with LVs from control WT (n = 10) and candesartan-treated WT (n = 8) mice. The GAPDH gene was used as an internal control. Figure S3. Current kinetics of Ito (A and C) and IKur(B and D). Time to peak (A and B) and decay time (100 to 50% of peak) (C and D) are plotted against membrane capacitance. Cm was poorly correlated with current kinetics of Ito or IKur. Figure S4. Typical images of myocytes obtained with 60× objective lens. A. WT, B. Candesartan treated DCM. C. Control DCM. (PDF)补充图S1. 肼屈嗪对心电图（ECG, Electrocardiogram）数据的影响。A. 野生型（WT, Wild Type）及肼屈嗪处理的扩张型心肌病（DCM, Dilated Cardiomyopathy）小鼠的典型心电图波形。B. QRS波（上图）与校正QT间期（QTc, Corrected QT interval）时长的对比。野生型、未处理对照组及坎地沙坦处理的扩张型心肌病小鼠的数据与图2B一致。肼屈嗪处理组样本量n=7。*P†P 补充图S2. 坎地沙坦处理对野生型左心室编码钾离子通道及其辅助亚基的mRNA表达水平的影响。采用对照野生型小鼠（n=10）与坎地沙坦处理的野生型小鼠（n=8）的左心室组织进行实时定量聚合酶链式反应分析，以甘油醛-3-磷酸脱氢酶（GAPDH）基因作为内参基因。 补充图S3. 瞬时外向钾电流（Ito）与超快延迟整流钾电流（IKur）的电流动力学特性。将达峰时间（A、B）与衰减时间（峰值的100%至50%，C、D）分别对膜电容（Cm）作图。结果显示膜电容与Ito或IKur的电流动力学相关性较弱。 补充图S4. 采用60倍物镜拍摄的心肌细胞典型图像。A. 野生型组；B. 坎地沙坦处理的扩张型心肌病组；C. 扩张型心肌病对照组。（PDF格式）