Hepatocyte RNA-seq analysis in liver-specific XBP1 knockout mice fed a high fat sugar diet

X-box binding protein 1 (XBP1) is a key component of the unfolded protein response (UPR) and plays important roles in the pathogenesis of nonalcoholic fatty liver diseases (NAFLD). Mice with liver-specific XBP1 deletion developed great liver injury and fibrosis in a dietary model of NAFLD. This project is to investigate hepatocyte-specific transcriptome profiling in XBP1-deficient mice fed a high fat sugar diet. Overall design: Liver-specific Xbp1 deficient mice or flox controls were fed either regular chow or a high fat sugar (HFS) diet for up to 6 weeks. Hepatocytes were isolated at the conclusion of the experiments and RNA was extracted with Trizol. Samples in HFS diet cohorts (n=6) were pooled into three replicates.