Dinoflagellate SPX domain-containing protein sequences

We used BLAST (basic local alignment search tool) analysis to identify SPXc genes within dinoflagellate genomes and transcriptomes. The analysis involved querying molecular databases such as Uniprot (https://www.uniprot.org/ (accessed on 5 May 2024) and SAGER (Symbiodiniaceae and algae genomic database) [24] with SPX gene sequences from the plant model Arabidopsis thaliana and PFAM seed sequences (PF03105) as queries. Hits with significant sequence identity (E-value < 1 × 10−5) were initially identified as potential homologs of SPXc genes. The presence of putative SPX domains was further verified using CDD in the NCBI, Pfam, and SMART databases (E-value < 1 × 10−5), and sequences with SPX domains identified in at least two databases were retained for subsequent analysis.