Curvilinear velocity and path linearity of rainbow trout spermatozoa activated in the presence of ovarian fluid and other conditions.zip

The data represent the motility traits (curvilinear velocity and path linearity) of rainbow trout (<i>Oncorhynchus mykiss</i>) spermatozoa in various activating solutions: ovarian fluid, its dilutions and molecular weight fractions, water and salines with different osmolarity and calcium ion content. Video records of motile cells were analyzed using ImageJ software and the following plugins: CASA and CASA modified for multiple analyses. The assessment of motility parameters in different activation media was conducted in triplicate for 26 males in water, ovarian fluid, and NaCl isotonic saline, 16 males in ovarian fluid dilutions, 12 males in media with varying osmolarity based on sodium chloride, and 11 males in MWCO fractions of ovarian fluid. Sperm samples from 8 males were used for experiments with the combined effects of Ca²⁺ concentrations and osmolarity.<br>Dataset belongs to the paper: Kholodnyy, V.; Dzyuba, B.; Rodina, M.; Bloomfield-Gadêlha, H.; Yoshida, M.; Cosson, J.; Boryshpolets, S. Does the Rainbow Trout Ovarian Fluid Promote the Spermatozoon on Its Way to the Egg? <i>Int. J. Mol. Sci. </i><b>2021</b>, <i>22</i>