Generation of recombinant plasmids by Golden Gate and Gibson cloning.

Vectors were generated by inserting specific inserts into already existing backbones. For the cloning, single to multi-fragment Gibson or Golden Gate assemblies were performed as indicated. Plasmids were either generated in the course of this work (“This paper”), described previously (Morales et al. 2023), or kindly provided by external parties. gDNA from a pLEXSY strain was obtained from Jena Bioscience. (XLSX)载体通过将特异性插入片段插入至已有的载体骨架序列中构建得到。分子克隆实验中，按照实验指示开展单片段至多片段的吉布森（Gibson）组装或金门（Golden Gate）组装。质粒的来源分为三类：本研究工作中构建（即“本论文”所述）、既往文献报道（Morales等人，2023年），以及由外部合作方惠赠。pLEXSY菌株的基因组DNA（gDNA）购自耶拿生物科技公司（Jena Bioscience）。(XLSX)