Generation of recombinant plasmids by Golden Gate and Gibson cloning.

Vectors were generated by inserting specific inserts into already existing backbones. For the cloning, single to multi-fragment Gibson or Golden Gate assemblies were performed as indicated. Plasmids were either generated in the course of this work (“This paper”), described previously (Morales et al. 2023), or kindly provided by external parties. gDNA from a pLEXSY strain was obtained from Jena Bioscience. (XLSX)