8H5 reactivity and dissociation constants of p125 and related 12mer peptides.

†Residues which could be randomly substituted shown in parenthesis.‡ The 8H5 reactivity of phage bearing p125 and the related 12mer peptides was determined by titration as in Figure 1 and expressed in titer defined as the phage dilution yielding an OD value of 1.5 (Tod1.5).*8H5 reactivity of the respective peptides was determined as in Figure 2 and expressed in blocking dose, defined as amount of the peptide that block binding of 8H5 to the indicated strain of the H5N1 avian influenza virus by 50%.§ Affinity of the peptides for 8H5 was determined by surface plasma resonance using BiaCore and expressed as dissociation constant (Kd).