8H5 reactivity and dissociation constants of p125 and related 12mer peptides.

†Residues which could be randomly substituted shown in parenthesis.‡ The 8H5 reactivity of phage bearing p125 and the related 12mer peptides was determined by titration as in Figure 1 and expressed in titer defined as the phage dilution yielding an OD value of 1.5 (Tod1.5).*8H5 reactivity of the respective peptides was determined as in Figure 2 and expressed in blocking dose, defined as amount of the peptide that block binding of 8H5 to the indicated strain of the H5N1 avian influenza virus by 50%.§ Affinity of the peptides for 8H5 was determined by surface plasma resonance using BiaCore and expressed as dissociation constant (Kd).† 括号内标注的为可随机替换的氨基酸残基（residues）。 ‡ 携带p125及相关12聚体肽（12mer peptides）的噬菌体的8H5反应活性，参照图1所示方法通过滴定法测定，以滴度（Tod1.5）表示，即光密度（OD，Optical Density）值达到1.5时的噬菌体稀释倍数。 * 各肽段的8H5反应活性参照图2测定，以阻断剂量表示，即能将8H5与指定H5N1禽流感病毒毒株的结合阻断50%的肽段用量。 § 肽段与8H5的亲和力采用表面等离子体共振（surface plasma resonance，SPR）技术，通过BiaCore仪器测定，并以解离常数（Kd）表示。