Data from: The translational landscape of the splicing factor SRSF1 and its role in mitosis

The shuttling Serine/Arginine rich (SR) protein SRSF1 (previously known as SF2/ASF) is a splicing regulator that also activates translation in the cytoplasm. In order to dissect the gene network that is translationally regulated by SRSF1, we performed a high-throughput deep sequencing analysis of polysomal fractions in cells overexpressing SRSF1. We identified approximately 1,500 mRNAs that are translational targets of SRSF1. These include mRNAs encoding proteins involved in cell cycle regulation, such as spindle, kinetochore and M phase proteins, which are essential for accurate chromosome segregation. Indeed, we show that translational activity of SRSF1 is required for normal mitotic progression. Furthermore, we found that mRNAs that display alternative splicing changes upon SRSF1 overexpression are also its translational targets; strongly suggesting that SRSF1 couples pre-mRNA splicing and translation. These data provide insights on the complex role of SRSF1 in the control of gene expression at multiple levels and its implications in cancer.

穿梭型富含丝氨酸/精氨酸（Serine/Arginine-rich, SR）蛋白SRSF1（曾用名SF2/ASF）是一类剪接调控因子，同时可在细胞质中激活翻译过程。为解析受SRSF1翻译调控的基因网络，我们对过表达SRSF1的细胞中的多聚核糖体组分开展了高通量深度测序分析，共鉴定出约1500个SRSF1的翻译调控靶mRNA。这些靶mRNA编码的蛋白参与细胞周期调控，包括纺锤体蛋白、动粒蛋白与M期蛋白等，而这些蛋白对精准染色体分离至关重要。实验证实，SRSF1的翻译活性对维持正常有丝分裂进程必不可少。此外，我们发现经SRSF1过表达后发生可变剪接改变的mRNA同样属于其翻译靶标，这强烈提示SRSF1可偶联前体mRNA剪接与翻译过程。本研究数据揭示了SRSF1在多层面调控基因表达中的复杂作用，及其在癌症发生中的潜在意义。