Specificity of fluorophore – streptavidin detection of biotinylated total human DNA probe in metaphase chromosomes and photostability ()

() Control (no fluorophore – streptavidin conjugate); () streptavidin – Qdot 605 detection of chromosome 1q12 region (vertical and horizontal arrows); () Texas Red – streptavidin detection of biotinylated DNA hybridized to 1q12 (vertical arrows) and () FITC – streptavidin detection of 1q12 sites (vertical arrows). Bar in panel () is 10 μm. () Signal decay upon continuous illumination with fluorescence microscope/mercury illumination in metaphase chromosome band 1q12 during 2 h continuous illumination. Red is Qdot 605, green is Texas Red, and blue is FITC. () Total intensity of whole interphase nuclei during 120 ms illumination (blue bars) and background (red bars). = 3 cells in each. Copyright © 2004 Oxford University Press. Xiao Y, Barker PE. 2004. Semiconductor nanocrystal probes for human metaphase chromosomes. , 32:e28. Reproduced with permission.<b>Copyright information:</b>Taken from "Potential clinical applications of quantum dots"International Journal of Nanomedicine 2008;3(2):151-167.Published online Jan 2008PMCID:PMC2527673.© 2008 Dove Medical Press Limited. All rights reserved(1) 对照组（无荧光基团⁠–⁠链霉亲和素偶联物）；(2) 链霉亲和素⁠–⁠量子点605（Qdot 605）检测1q12染色体区域（以垂直及水平箭头标注）；(3) 德克萨斯红⁠–⁠链霉亲和素检测与1q12位点杂交的生物素化DNA（以垂直箭头标注）；(4) 异硫氰酸荧光素（FITC）⁠–⁠链霉亲和素检测1q12结合位点（以垂直箭头标注）。本系列所有面板的标尺长度为10 μm。(5) 中期染色体1q12条带经荧光显微镜汞灯持续照射2小时后的信号衰减情况，其中红色信号对应量子点605（Qdot 605），绿色信号对应德克萨斯红，蓝色信号对应异硫氰酸荧光素（FITC）。(6) 120 ms照射条件下完整间期细胞核的总荧光强度（蓝色柱）与背景荧光强度（红色柱），每组包含3个细胞。 © 2004 牛津大学出版社。作者为Xiao Y、Barker PE，2004年发表文章《Semiconductor nanocrystal probes for human metaphase chromosomes》，载于第32卷，e28。经许可转载。 <b>Copyright information:</b> 本文摘自"Potential clinical applications of quantum dots"，《国际纳米医学杂志》（*International Journal of Nanomedicine*）2008年；3(2):151-167，2008年1月在线发表，PMCID:PMC2527673。© 2008 Dove Medical Press Limited。保留所有权利。