Resistance assay values.

The continuous use of Di(2-ethylhexyl) phthalate (DEHP) in plastic products turns it into a ubiquitous contaminant in the environment. However, DEHP can cause harm to human beings, wildlife, and ecosystems due to its estrogenicity and toxicity. Thus, finding an efficient approach to removing this contaminant from the environment is crucial. The present study aimed to prospect and characterize a bacterial consortium (MP001) isolated from a neotropical mangrove for DEHP bioremediation. A laboratory experiment was performed with environmentally relevant DEHP concentrations (0.05, 0.09, 0.19, 0.38, 0.75, 1.50, 3.00, and 6.00 mg L-1) to determine the consortium resistance to this contaminant and high-throughput sequencing was accomplished to assess the bacterial composition, diversity, and potential ecological function of consortium MP001. The consortium MP001 presented a significant biomass increase throughout short-term incubations with increasing concentrations of DEHP (GLMs, pParaclostridium sp. (78.99%) and Bacillus sp. (10.73%). After 48 h of consortia exposure to DEHP, the bacterial population changed to Paraclostridium (50.00%), Staphylococcus sp. (12.72%), Staphylococcus epidermidis (10.40%) and Bacillus sp. (17.63%). In the negative control, the bacteria community was composed of Paraclostridium sp. (54.02%), Pseudomonas stutzeri (19.44%), and Staphylococcus sp. (11.97%). The alpha diversity of the MP001 consortium was not significant (Kruskall-Wallis; p > 0.05), and no significant difference was found between the DEHP treatment and the negative control. Furthermore, the potential ecological function found in the consortium MP001 with higher potential for application in bioremediation purposes was fermentation. The results found in this study highlight the potential of a bacterial consortium to be used in the bioremediation of DEHP-contaminated aquatic environments.邻苯二甲酸二(2-乙基己基)酯（Di(2-ethylhexyl) phthalate, DEHP）在塑料制品中的持续使用，使其成为环境中广泛分布的污染物。由于其雌激素活性与毒性，DEHP会对人类、野生动物及生态系统造成损害，因此开发高效的环境污染物去除方法具有重要意义。 本研究旨在探究从新热带红树林中分离得到的细菌联合体MP001，并对其用于DEHP生物修复的潜力进行表征。本研究设置了环境相关浓度梯度（0.05、0.09、0.19、0.38、0.75、1.50、3.00及6.00 mg L⁻¹）的DEHP暴露实验室实验，以测定该联合体对该污染物的耐受性；同时通过高通量测序（high-throughput sequencing）技术，解析MP001的细菌群落组成、多样性及潜在生态功能。 在短期培养过程中，随着DEHP浓度升高，MP001联合体的生物量显著提升（广义线性模型（Generalized Linear Models, GLMs），p<0.05）。群落组成分析显示，初始阶段该联合体以副梭菌属（Paraclostridium sp.，78.99%）和芽孢杆菌属（Bacillus sp.，10.73%）为优势类群。当联合体暴露于DEHP 48小时后，群落结构转变为副梭菌属（50.00%）、葡萄球菌属（Staphylococcus sp.，12.72%）、表皮葡萄球菌（Staphylococcus epidermidis，10.40%）及芽孢杆菌属（17.63%）。在阴性对照组中，细菌群落则由副梭菌属（54.02%）、施氏假单胞菌（Pseudomonas stutzeri，19.44%）及葡萄球菌属（11.97%）构成。 MP001联合体的α多样性无显著差异（克鲁斯卡尔-沃利斯检验（Kruskall-Wallis），p>0.05），且DEHP处理组与阴性对照组间无显著差异。进一步分析显示，该联合体中与生物修复应用潜力高度相关的潜在生态功能为发酵作用。本研究结果证实，细菌联合体MP001具备用于DEHP污染水生环境生物修复的良好潜力。