Multi-Omic Approach Associates Blood Methylome with Bronchodilator Drug Response in Pediatric Asthma: Summary statistics

We conducted an epigenome-wide association study of bronchodilator drug response (BDR) in a discovery and validation design. The discovery phase was focused on 221 African American children with asthma. The association between DNA methylation and BDR was conducted using the limma package correcting for age, sex, ancestry, and tissue heterogeneity. Summary statistics include the output from toptable limma function and CpG annotation (based on Illumina EPIC Manifest file v 1.0 B4) organized in the following columns: Probe: Probe ID Chr: chromosome Pos: genomic position based on GRCh37/hg19 Gene: Gene annotation based on Illumina EPIC Manifest file v 1.0 B4 logFC: estimate of the log2-fold-change corresponding to the effect or contrast SE: standard error AveExpr: average log2-expression for the probe over all arrays and channels t: moderated t-statistic P.value: raw p-value FDR: adjusted p-value by false discovery rate B: log-odds that the gene is differentially expressed Problem: Flagged potentially problematic probes我们采用发现-验证设计，开展了针对支气管舒张剂药物反应（BDR，bronchodilator drug response）的表观全基因组关联研究。发现阶段的研究对象为221名罹患哮喘的非裔美国儿童。本研究采用limma软件包开展DNA甲基化与BDR之间的关联分析，并校正了年龄、性别、祖先血统及组织异质性。汇总统计量包含limma的toptable函数输出结果，以及基于Illumina EPIC Manifest file v 1.0 B4的CpG位点注释信息，各列信息如下：Probe：探针ID；Chr：染色体；Pos：基于GRCh37/hg19的基因组位置；Gene：基于Illumina EPIC Manifest file v 1.0 B4的基因注释；logFC：对应效应或对比的log₂倍变化估计值；SE：标准误；AveExpr：该探针在所有阵列与通道中的平均log₂表达量；t：调节型t统计量；P.value：原始p值；FDR：经错误发现率校正的p值；B：基因存在差异表达的对数优势值；Problem：标记潜在问题的探针。