Article data A simple and unbiased method of analyzing lipid droplets combining Oil Red staining and confocal microscopy

Our method using confocal microscopy substantially enhanced the quality of lipid droplets (LD) images compared to phase contrast microscopy by eliminating out-of-focus light, increasing contrast, enhancing lipid droplet definition and increasing the sensitivity of LD quantification.

相较于相差显微镜（phase contrast microscopy），本研究采用的共聚焦显微镜（confocal microscopy）成像方法通过消除离焦光、提升图像对比度、增强脂滴（lipid droplets，LD）的轮廓清晰度，以及提高脂滴定量分析的灵敏度，大幅提升了脂滴图像的成像质量。