Data on proteins identified by TMT-labeled proteomics in alveolar type II epithelial cells

The objective of this study was to characterize differentially regulated proteins and biological processes in hydrogen-treated hyperoxic primary type II alveolar epithelial cells (AECIIs) to elucidate the protective mechanism of hydrogen using quantitative proteomics. AECIIs were divided into three groups that were cultured for 24 h in three different conditions: control (21% oxygen), hyperoxia (95% oxygen), and hyperoxia + hydrogen. The TMT labeling quantitative proteome technique was used to detect changes in the protein expression profile, and bioinformatics analysis was performed.本研究旨在通过定量蛋白质组学技术，对经氢气干预的高氧原代肺泡II型上皮细胞（type II alveolar epithelial cells, AECIIs）中的差异表达蛋白及生物学过程进行表征，以阐明氢气的保护作用机制。将AECIIs分为三组，分别在三种不同条件下培养24小时：对照组（21%氧气）、高氧组（95%氧气）以及高氧+氢气组。本研究采用串联质量标签（Tandem Mass Tag，TMT）标记定量蛋白质组学技术检测蛋白质表达谱的变化，并进行生物信息学分析。