Supplementary Material for: Interaction between Common Genetic Variants and Total Fat Intake on Low-Density Lipoprotein Peak Particle Diameter: A Genome-Wide Association Study

Background/Aim: Total fat intake has an important impact on the low-density lipoprotein (LDL) peak particle diameter (LDL-PPD) and may interact with nutrient-sensitive single nucleotide polymorphisms (SNPs). The objective was to examine whether there is suggestive evidence of SNP × dietary fat intake interaction effects influencing the LDL-PPD in the Quebec Family Study (QFS) in order to generate hypotheses to be tested in larger studies. Methods: SNPs from a genome-wide association study (GWAS) using Illumina Human610-Quad BeadChip, total fat intake derived from a 3-day weighted food record, and SNP × total fat intake interaction effects were examined on LDL-PPD in 541 QFS subjects. Results: The GWAS analyses 29 identified independent SNP × total fat intake interaction effects on the LDL-PPD at p -5, including SNPs in the following genes: ABCG2, CPA3, FNBP1, KCNQ3, NBAS, NCALD, OPRL1, NKAIN2, SH3BGRL2, SOX5, and SUSD4. Conclusions: This observational study suggests that multiple SNPs interact with dietary fat intake to influence variation in the LDL-PPD.研究背景与目的：总脂肪摄入对低密度脂蛋白（low-density lipoprotein, LDL）峰值颗粒直径（LDL-PPD）具有重要影响，且可能与营养敏感性单核苷酸多态性（single nucleotide polymorphisms, SNPs）存在交互作用。本研究旨在魁北克家庭研究（Quebec Family Study, QFS）中，探究是否存在可提示性证据证明单核苷酸多态性与膳食脂肪摄入的交互作用会对LDL-PPD产生影响，以期生成可在更大规模研究中验证的假说。 方法：本研究纳入541名魁北克家庭研究（QFS）受试者，采用基于Illumina Human610-Quad基因芯片的全基因组关联研究（genome-wide association study, GWAS）获取单核苷酸多态性数据，通过3天加权食物记录法获取总脂肪摄入数据，并分析单核苷酸多态性与总脂肪摄入的交互作用对LDL-PPD的影响。 结果：全基因组关联研究分析显示，在P<10^-5的显著性水平下，共鉴定出29个独立的单核苷酸多态性与总脂肪摄入的交互作用位点，这些位点所在的基因包括ABCG2、CPA3、FNBP1、KCNQ3、NBAS、NCALD、OPRL1、NKAIN2、SH3BGRL2、SOX5及SUSD4。 结论：本观察性研究表明，多个单核苷酸多态性可与膳食脂肪摄入产生交互作用，进而影响LDL-PPD的水平变异。