MicroRNA sequencing for platinum-resistant high-grade serous ovarian carcinoma

Ovarian cancer is one of the leading causes of death in females in the world. High-grade serous ovarian carcinoma (HGSOC) is the most common histological subtype, and the platinum-resistance is a clinical challenge, In this study, we investigated the microRNA (miRNA) profiles of platinum-resistant and platinum-sensitive HGSOC. Overall design: Archival fresh frozen samples at the Hyogo Prefectural Cancer Center Biobank were used for analysis. Total RNA was extracted from the samples, and small RNA libraries were prepared using the NEBNext Multiplex Small RNA Library Prep Set for Illumina (New England Biolabs, Ipswich, MA). Finally, single-end reads were performed on the Illumina MiSeq.