Structure of the Nt domain of mouse NBCe1 by homology modeling

For the generation of the Nt model for NBCe1, a pair-wise alignment of the Nt region (residues 82-381) of mouse NBCe1-B (NCBI accession NP_061230.2) and the homologous portion of human NDCBE Nt (NCBI accession AAY79176) was generated by SWISS-MODEL. The overall identity is 54.55% between the two sequences. The alignment was used for structural modeling with the crystal structure of human NDCBE Nt (PDB ID: 5JHO) as the template. Structural assessment shows that the simulated model of NBCe1 Nt had QMEAN value −3.25, Cβ value −3.11, solvation value −0.62, torsion value −2.54, and scored 1.86 by using a MolProbity approach.