Microbial genome collection of aerobic granular sludge cultivated in sequential batch reactor using different carbon source mixtures

Abstract Aerobic granular sludge was cultivated in a sequencing batch reactor (SRB) fed with either volatile fatty acids (VFA), a mixture of complex monomeric substrates (CM), or a mixture of complex polymeric substrates (CP). Samples were taken during periods where the SBR was operated with the different carbon source mixtures: VFA (day 71), CM (day 322 and 427), and CP (day 740). Metagenomic DNA was sequenced and this study utilizes the dataset PRJEB38840 (Adler et al 2022 Sfam) to construct Metagenome-assembled genomes (MAGs) using two distinct workflows (A and B), making a in-house genome collection of 759 MAGs in total, from which 331 remained after dereplication step. Data including metagenomic assemblies, MAGs and its associated metadata are added to this Zenodo collection.摘要 本研究在序批式反应器（Sequencing Batch Reactor，缩写SBR）中培养好氧颗粒污泥（Aerobic granular sludge），分别以挥发性脂肪酸（Volatile Fatty Acids，缩写VFA）、复杂单体底物混合物（Complex Monomeric Substrates，缩写CM）及复杂聚合底物混合物（Complex Polymeric Substrates，缩写CP）作为进水碳源。在反应器采用不同碳源基质运行的阶段采集样品：VFA组（第71天）、CM组（第322天与第427天）以及CP组（第740天）。对宏基因组DNA进行测序后，本研究利用Adler等（2022）发布的数据集PRJEB38840（标注为Sfam），采用两种独立的分析流程（流程A与流程B）构建宏基因组组装基因组（Metagenome-assembled Genomes，缩写MAGs），最终得到总计759个MAGs的自建基因组集合，经去冗余步骤后保留331个MAGs。包含宏基因组组装结果、MAGs及其相关元数据的数据集已上传至该Zenodo集合。