Simple oligonucleotide-based multiplexing of single-cell chromatin accessibility

we reported the development of an accurate, easy-to-use and scalable system (SNuBar) for multiplexing single cell ATAC or multi-omics (ATAC&RNA) assays, and applied this method to multiplex therapy combinations in a lung cancer mouse model and macro-spatial regions of normal breast tissues and samples. SNuBar multiplexes scATAC-seq and scATAC&RNA-seq with unmodified oligonucleotides, identfies treatment-induced chromatin dynamics in 28 mice with lung tumors, profiles epigenetic heterogeneity of 32 spatial regions in normal breast tissues, identifies TFs that regulated breast cell type identities.