The effects of various levels of drought and heat on the rice shoot transcriptome. The effects of various levels of drought and heat on the rice shoot transcriptome

To investigate the relative effects of drought and heat on the rice (Oryza sativa) transcriptome, we subjected rice seedlings to 3 levels of long-term drought or 2 levels of short-term heat stock Overall design: Rice was grown for 10 days in 3 water levels: wet/control (3g/g SWC), mild drought (1.5g/g SWC) or severe drought (1.25g/g SWC). At 10 days, additional wet-condition plants were subjected to 30min or 60min heat shock. 3 reps of each condition above were collected at midday (6hr after dawn) for RNA-sequencing.