scRNAseq for characterization of Kitl-expressing stromal cells in ear skin

In this experiment, we want to identify which cell types in the ear skin express Kitl and characterize these cell types. For this, ears from two KitlGFP mice (18 weeks old littermate males from the same cage) were isolated and digested to obtain a single cell suspension. GFP positive, endothelial cell negative (CD31-) cells that were viable (DAPI-) were subsequently sorted in 2% BSA in PBS. scRNAseq was then performed.