Cryopreservation of semen from goats fed a diet supplemented with flaxseed

ABSTRACT The objective of this study was to evaluate the effects of the inclusion of flaxseed in the diet of male goats on the resistance of semen to the cryopreservation process. Sixteen males were distributed in four groups and fed a diet supplemented with 0, 4, 8, and 12% of flaxseed for a period of 60 days. Only the ejaculates that presented motility and vigor above 70% and 3, respectively, were sent through the cryopreservation process. After thawing, the semen was evaluated through thermo resistance, hypoosmotic, and acrossomal integrity tests. The data were submitted to analysis of variance and regression at 5% of significance. There was positive quadratic behavior for motility after 60, 120, and 180 min in the thermoresistance test (TTR), and positive quadratic behavior for sperm vigor after thawing after 120 and 180 min in the TTR (P摘要 本研究旨在评估在公山羊日粮中添加亚麻籽（flaxseed）对精液（semen）抗冷冻保存（cryopreservation）能力的影响。选取16只公山羊，随机分为4组，分别饲喂添加0%、4%、8%、12%亚麻籽的日粮，试验周期为60天。仅选取精子活力（motility）≥70%、精子活率（vigor）≥3级的精液样本进行冷冻保存处理。解冻后，通过耐热试验（thermoresistance test, TTR）、低渗肿胀试验及顶体完整性试验（acrosomal integrity test）对精液进行检测评估。采用显著性水平为5%的方差分析与回归分析对试验数据进行统计处理。耐热试验（TTR）结果显示，精液活力在60、120、180分钟时均呈现正二次变化趋势；解冻后精子活率在TTR试验的120、180分钟时同样呈现正二次变化趋势（P