Gene expression profile at single cell level of mouse tibialis anterior muscle cells from young and aged mice treated with or without cardiotoxin (CTX)

The findings show that skeletal muscle tissue during regeneration contains a variety of macrophage subpopulations, whose compositional proportions and functions change with age. Single-cell RNA sequencing (scRNA-seq) was used to analyse intercellular communication between proliferating skeletal muscle stem cells and macrophage subpopulations. Overall design: Live (7AAD-negative) leukocytes (CD45-positive), Fibro-adipogenic progenitors (Ly6A/E-positive), endothelial cells (CD31-positive), and skeletal muscle stem cells (CD106-positive) were sorted from mouse anterior tibial muscle tissue on days 0 and 3 of regeneration by fluorescence-activated cell sorting (FACS) and processed for droplet-based scRNA-seq.