Angiotensin Converting Enzyme (ACE) expression in microglia reduces amyloid β deposition and neurodegeneration by increasing SYK signaling and endolysosomal trafficking

Angiotensin Converting Enzyme (ACE) has been studied for its role in the regulation of myeloid cell bactericidal and antitumor activity. Here, we generated a mouse model with Tamoxifen-dependent ACE expression and Cx3CR1-Cre control. These mice were crossed to 5xFAD and 9 month forebrains were harvested and FFPE processed to assess the ACE-dependent changes in amyloid-reactive microglia. Paraffin tissue blocks were faced until matching anatomical regions were visible across all blocks and 3 hippocampal donor cores (1mm diameter) were extracted from each block and placed into a recipient tissue microarray block using a GrandMaster TMA (3D Histotechnology, Hungary) instrument