Summary of de novo YH genome assembly.

The data from the YH project was used for the contig and initial scaffold assembly. Then, the long-range PE data were added step by step for scaffold construction. Genome coverage and gene coverage was calculated using the NCBI build 37 and RefSeq gene set as reference, respectively. The X and Y chromosomes were excluded while calculating genome coverage and gene coverage. For calculation of scaffold N50, N90 and total length, the intra-scaffold gaps were included.本研究使用YH项目（YH Project）的数据完成了重叠群（contig）与初始支架（scaffold）的组装。随后逐步添加长程配对末端（PE, Pair-End）测序数据以完成支架的构建。分别以NCBI参考基因组组装版本37（NCBI build 37）与RefSeq基因集作为参照，计算基因组覆盖度与基因覆盖度；在计算该两类覆盖度时，需排除X染色体与Y染色体。在计算支架N50、N90及总长度时，需将支架内部的间隙（gaps）纳入统计范围。