Hellbender skin transcriptome metadata

In this study, we explored the efficacy of Bd vaccination on hellbender salamanders and functionally characterized host, host-associated microbiome, and pathogen throughout immunization and infection. We compared the function of hellbender skin, the skin microbiome, and Bd with a metatranscriptome approach at four experimental time points, (i) pre-vaccination and pre-infection, (ii) post-vaccination and pre-infection, (iii) early infection, and (iv) late infection.本研究探究了Bd（蛙壶菌）疫苗对隐鳃鲵（hellbender salamanders）的接种效力，并对免疫接种与感染全过程中的宿主、宿主相关微生物组及病原体开展功能表征。本研究采用宏转录组学（metatranscriptome）方法，在四个实验时间点对隐鳃鲵皮肤、皮肤微生物组及Bd的功能进行比较分析，四个时间点分别为：(i) 疫苗接种前且感染前，(ii) 疫苗接种后且感染前，(iii) 感染早期，(iv) 感染晚期。