Supplementary data from: Assessing the structural boundaries of broadly reactive antibody interactions with diverse H3 influenza hemagglutinin proteins

Influenza virus infections are an ongoing seasonal disease burden and persistent pandemic threat. Vaccine antigens that can elicit or recall broadly neutralizing antibodies are a high priority for the development of next-generation influenza vaccines to reduce the impact of antigenic drift on vaccine efficacy. Ideally, broadly neutralizing antibodies should be resilient to changes in viral antigens over time. This dataset contains raw and processed biolayer interferometry data of the binding characteristics of three antibodies (TJ5-1, TJ5-13, and #1664) with influenza H3 antigens representing almost 50 years of virus evolution. In addition, AlphaFold3 generated models of the A/Tasmania/503/2020 HA are included in the absence of publicly available models of related H3 structures., The BLI data was collected on an Octet Red384 instrument in the Macromolecular Structure Funtion Core Facility. Data processing was performed using the Octet Data Analysis HT software v7. Molecular models were obtained from the AlphaFold3 server (https://alphafoldserver.com). , , # Supplementary data from: Assessing the structural boundaries of broadly reactive antibody interactions with diverse H3 influenza hemagglutinin proteins

[https://doi.org/10.5061/dryad.tmpg4f57v](https://doi.org/10.5061/dryad.tmpg4f57v)

## Description of the data and file structure

Supplemental data for the manuscript \"Assessing the structural boundaries of broadly reactive antibody interactions with diverse H3 influenza hemagglutinin proteins\". Raw and processed data are included from biolayer interferometry experiments of broadly neutralizing antibodies against a diverse range of hemagglutinin proteins derived from H3N2 viruses. Correspondence should be addressed to Rebecca DuBois ([[e-mail]](mailto:[e-mail])).

### Files and variables

#### File: Dryad_Deposition.zip

**Description:** Compressed archive containing manuscript supplemental data.

#### Folder: Raw_Data

**Description:** Folder containing Microsoft Excel files with raw data for biolayer interferomet...,

流感病毒感染是持续存在的季节性疾病负担与长期存在的大流行威胁。能够诱导或召回广谱中和抗体的疫苗抗原，是下一代流感疫苗研发的重中之重，以降低抗原漂移对疫苗效力的影响。理想状态下，广谱中和抗体应能抵御病毒抗原随时间推移发生的变异。本数据集包含三种抗体（TJ5-1、TJ5-13及#1664）与覆盖近50年病毒演化历程的H3型流感抗原结合特性的原始及处理后的生物层干涉测量（biolayer interferometry, BLI）数据。此外，由于尚无公开可用的相关H3结构模型，本数据集还纳入了AlphaFold3生成的A/Tasmania/503/2020 HA模型。
生物层干涉测量数据采集于大分子结构与功能核心设施的Octet Red384仪器。数据处理采用Octet Data Analysis HT v7软件完成。分子模型获取自AlphaFold3服务器（https://alphafoldserver.com）。

本补充数据来自论文《广谱反应性抗体与多样H3型流感血凝素蛋白相互作用的结构边界评估》（Assessing the structural boundaries of broadly reactive antibody interactions with diverse H3 influenza hemagglutinin proteins），对应DOI：https://doi.org/10.5061/dryad.tmpg4f57v。

## 数据与文件结构说明
本数据集为上述论文的补充数据，包含针对源自H3N2病毒的多种血凝素蛋白的广谱中和抗体所开展的生物层干涉测量实验的原始与处理后数据。通信联系请致Rebecca DuBois（邮箱：[e-mail]）。

### 文件与变量
#### 文件：Dryad_Deposition.zip
**描述：** 包含论文补充数据的压缩归档文件。
#### 文件夹：Raw_Data
**描述：** 存放生物层干涉测量原始数据的Microsoft Excel文件的文件夹，内容未完整展示。